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Influence of Hyaluronic Acid on the Different Levels of Lysozyme and Peroxidase in the Aspects of Candidacidal Activities
J Oral Med Pain 2018;43:1-7
Published online March 30, 2018
© 2018 Korean Academy of Orofacial Pain and Oral Medicine

Jihoon Kim1, Yoon-Young Kim1, Ji-Youn Chang1, Hong-Seop Kho1,2

1Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Korea
2Institute on Aging Seoul National University, Seoul, Korea
Correspondence to: Hong-Seop Kho
Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea
Tel: +82-2-2072-3989
Fax: +82-2-744-9135
E-mail: hkho@snu.ac.kr
Received February 3, 2018; Revised March 5, 2018; Accepted March 7, 2018.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Purpose: The purpose of the study was to investigate the influences of hyaluronic acid on the candidacidal activities of lysozyme, the peroxidase system, and the glucose oxidase-mediated peroxidase (GO-PO) system at different concentrations of antimicrobial enzymes.
Methods: Hyaluronic acid was used at a final concentration of 0.5 mg/mL. Hen egg-white lysozyme (HEWL) was used at concentrations ranging from 10 to 100 μg/mL. The peroxidase system included bovine lactoperoxidase (bLPO), potassium thiocyanate (KSCN, 1 mM), and hydrogen peroxide (100 μM). The GO-PO system included bLPO, KSCN (1 mM), glucose oxidase (10 units/mL), and glucose (30 μg/mL). The final concentration of bLPO in the peroxidase and GO-PO systems ranged from 12.5 to 100 μg/mL. Candida albicans strains ATCC 10231, 11006, and 18804 were utilized. Candidacidal activities of antimicrobials and the influence of hyaluronic acid on their candidacidal activities were determined based on colony forming units.
Results: Candidacidal activities of the peroxidase and GO-PO systems increased with increasing concentrations of bLPO. This tendency was the same in the presence or absence of hyaluronic acid. Candidacidal activity of HEWL was not significantly concentration-dependent. Candidacidal activities of the GO-PO system were higher than those of the corresponding peroxidase system. Candidacidal activity was inhibited in the presence of hyaluronic acid in the following order: HEWL, the peroxidase system, and the GO-PO system.
Conclusions: Hyaluronic acid inhibited the candidacidal activities of HEWL, the peroxidase system, and the GO-PO system. The GO-PO system exhibited better candidacidal activity than HEWL and the peroxidase system both in the presence and absence of hyaluronic acid.
Keywords : Candidacidal activity; Hyaluronic acid; Lysozyme; Peroxidase


June 2018, 43 (2)