search for




 

The Effect of Dilution with 10% Dextrose Solution on the Potency of Botulinum Toxin A
J Oral Med Pain 2019;44:11-15
Published online March 30, 2019;  https://doi.org/10.14476/jomp.2019.44.1.11
© 2019 Korean Academy of Orofacial Pain and Oral Medicine

Jong Wook Ham1, Jeong-Seung Kwon2, Eunae Sandra Cho3, Jong Hoon Choi2,4

1Department of Orthodontics and Cosmetic Dentistry, Dr. Ham’s Dental and Botulinum Toxin Clinic, Seoul, Korea, 2Department of Orofacial Pain and Oral Medicine, Yonsei Dental Hospital, Yonsei University College of Dentistry, Seoul, Korea, 3Department of Oral Pathology, Oral Cancer Research Institute, Yonsei University College of Dentistry, Seoul, Korea, 4Oral Science Research Center, Yonsei University College of Dentistry, Seoul, Korea
Correspondence to: Jong Hoon Choi
Oral Science Research Center, Yonsei University College of Dentistry, Department of Orofacial Pain and Oral Medicine, Dental Hospital of Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemungu, Seoul 03722, Korea, Tel: +82-2-2228-3113 Fax: +82-2-393-5673, E-mail: jhchoij@yuhs.ac, https://orcid.org/0000-0003-3211-3619
Received January 18, 2019; Revised February 2, 2019; Accepted February 2, 2019.
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Purpose: The aim of this study was to compare the potency-stabilizing effects of two different diluents of botulinum toxin A (10% dextrose solution and 0.9% saline).
Methods: A mouse lethality bioassay was undertaken. Ninety mice were divided into experimental and control groups which received varying dosages in subgroups of 10. The experimental group was injected with botulinum toxin A diluted with 10% dextrose solution and the control group was injected with botulinum toxin A diluted with 0.9% saline. A 72 hours after intraperitoneal injection, the number of dead mice was counted to confirm median lethal dose (LD50) of each group.
Results: The value of LD50 in the experimental group was approximately 0.131 mL (1.31 U) and the value of LD50 in the control group was approximately 0.107 mL (1.07 U). The potency preservation rate of the experimental group was estimated to be 93.5% and that of the control group was estimated to be 76.3%.
Conclusions: Dilution with 10% dextrose solution displayed less potency loss than 0.9% saline.
Keywords : Botulinum toxins, Type A; Glucose; Saline solution


March 2019, 44 (1)